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Image Search Results
Journal: Infection and Immunity
Article Title: Staphylococcus aureus Colonization Induces Strain-Specific Suppression of Interleukin-17
doi: 10.1128/IAI.00834-17
Figure Lengend Snippet: Suppressive cytokine responses to endogenous and exogenous S. aureus strains. IL-19 mRNA expression (a) and protein secretion (b) in response to endogenous strains (SA*) were increased compared to those in response to exogenous ones (SA) (n = 18 and 7; P = 0.05 and 0.03). No difference between expression levels and protein secretion levels of IL-27 (c, d) (n = 15,9; P = 0.42; 0.43) or Treg-associated factors FOXP3 (e) (n = 12; P = 0.51) and IL-10 (f, g) (n = 8,12; P = 0.29, 0.95) was seen. In each experiment, one endogenous strain was tested and compared to at least two exogenous strains. Each data point represents the FOXP3 or cytokine response of one carrier to one strain in one experiment. Lines indicate the geometric means of all of the data points in a column. (h) Ratios of cytokine responses to exogenous versus endogenous strains. When the P value is >0.2, ns indicates nonsignificance.
Article Snippet: A
Techniques: Expressing
Journal: Infection and Immunity
Article Title: Staphylococcus aureus Colonization Induces Strain-Specific Suppression of Interleukin-17
doi: 10.1128/IAI.00834-17
Figure Lengend Snippet: IL-17 expression in response to recombinant IL-19 and an anti-IL-19 antibody. (a) Addition of recombinant IL-19 to PBMCs caused a small, insignificant decrease in IL-17 mRNA expression. Addition of recombinant IL-19 at 10 or 100 pg/ml to PBMC cultures stimulated with an exogenous strain decreased IL-17 mRNA expression compared to that in exogenous-strain-stimulated cultures with no addition (P < 0.0001). (b) Addition of IL-19 antibodies to PBMCs caused an insignificant increase in IL-17 mRNA expression, and addition of IL-19 antibodies at 0.8 μg/ml to PBMC cultures stimulated with an endogenous strain resulted in an increased IL-17 mRNA response compared to that of the endogenous-strain-stimulated culture with no addition (P < 0.0001). ANOVA with Tukey's post hoc analysis was done on all repeats to determine differences in IL-17 mRNA expression. (c) mRNA expression of IL-17 and IL-19 over 48 h in PBMCs isolated from one carrier in response to an endogenous strain.
Article Snippet: A
Techniques: Expressing, Recombinant, Isolation